论文部分内容阅读
应用等离子辅助化学接枝方法在磷灰石-硅灰石生物活性玻璃(AW)的表面接枝精氨酸-甘氨酸-天冬氨酸(RGD)多肽。采用模拟体液浸泡方法研究了AW表面接枝RGD基团对材料体外矿化特性的影响,SEM检测结果表明,RGD多肽的引入有利于羟基磷灰石(HA)的沉积,能够增强RGD-AW复合材料的体外矿化能力,HA形貌为蠕虫状。材料-MG63细胞共培养实验以及材料新西兰成年大白兔体内植入实验的结果表明,表面化学接枝RGD多肽的RGD-AW复合材料能够显著地促进类成骨细胞的黏附和铺展,并且在2周、4周和8周时均能够加速新骨的生成及骨组织结构和功能的重建。
The plasma-assisted chemical grafting method was used to graft arginine-glycine-aspartic acid (RGD) on the surface of apatite-wollastonite bioactive glass (AW). The effect of grafting RGD groups on the surface mineralization of AW materials was studied by simulated body-fluid immersion method. The results of SEM showed that the introduction of RGD peptides was beneficial to the deposition of hydroxyapatite (HA) and RGD-AW complex In vitro mineralization of materials, HA morphology worm-like. MATERIAL-MG63 CELL CULTURE EXPERIMENT AND MATERIALS The results of in vivo implantation experiments in adult New Zealand white rabbits showed that the RGD-AW composite with RGD peptide grafted chemically on the surface could significantly promote osteoblast-like adhesion and spreading, , 4 weeks and 8 weeks can accelerate the formation of new bone and bone tissue structure and function of the reconstruction.