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目的:研究川芎嗪对人视网膜母细胞瘤(RB)SO-RB50细胞增殖的影响,并探讨其作用与趋化因子CXCR4表达水平之间的关系。方法:体外培养人SO-RB50细胞,经川芎嗪处理不同时间后,应用MTT法检测SO-RB50细胞的增殖抑制;采用荧光定量逆转录PCR和蛋白免疫印迹法分别从基因和蛋白质水平检测川芎嗪对SO-RB50细胞趋化因子受体CXCR4表达的影响,进而应用流式细胞仪检测SO-RB50细胞的细胞周期分布。结果:MTT结果显示川芎嗪对人SO-RB50细胞增殖有明显的抑制作用,与对照组比较差异具有统计学意义(P<0.01);细胞周期结果显示川芎嗪处理后的SO-RB50细胞G0/G1期增多,S期细胞减少,与对照组比较差异有统计学意义(P<0.01);通过荧光定量逆转录PCR及蛋白免疫印迹实验证明,川芎嗪能下调人SO-RB50细胞CXCR4的表达水平。结论:川芎嗪对人SO-RB50细胞的增殖有明显抑制作用,且能下调SO-RB50细胞中与肿瘤发生及生长密切相关的CXCR4表达水平。
OBJECTIVE: To study the effect of ligustrazine on the proliferation of human retinoblastoma (RB) SO-RB50 cells and to explore its relationship with the chemokine CXCR4 expression. Methods: Human SO-RB50 cells were cultured in vitro and treated with Ligustrazine for different time. MTT assay was used to detect the proliferation inhibition of SO-RB50 cells. Ligustrazine was detected by quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting On the expression of chemokine receptor CXCR4 in SO-RB50 cells, and then the flow cytometry was used to detect the cell cycle distribution of SO-RB50 cells. Results: The results of MTT showed that ligustrazine could significantly inhibit the proliferation of human SO-RB50 cells, and the difference was statistically significant (P <0.01). The results of cell cycle showed that ligustrazine-treated G0 / The expression of CXCR4 in human SO-RB50 cells was down-regulated by fluorescence quantitative reverse transcription polymerase chain reaction (RT-PCR) and western blotting . CONCLUSION: Ligustrazine can significantly inhibit the proliferation of human SO-RB50 cells and down-regulate the expression of CXCR4 in SO-RB50 cells, which is closely related to tumorigenesis and growth.