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黑色素瘤恶性程度高,目前尚无比较有效的防治手段。黑色素瘤表面存在大量的α-促黑激素(α-MSH)受体,由α-MSH和绿脓杆菌外毒素(作用于靶细胞肽链延伸因子Ⅱ使其失活而影响蛋白质合成,最终导致靶细胞死亡),构建成的基因重组免疫毒素将能特异地杀伤靶细胞一黑瘤细胞。本实验采用PCR技术,获得α-MSH和PE40(绿脓杆菌外毒素缺失细胞结合功能区域,从而避免免疫毒素
High degree of malignancy of melanoma, there is no effective prevention and treatment. A large number of α-MSH receptors exist on the surface of melanoma, which are caused by the inactivation of α-MSH and Pseudomonas exotoxin (acting on the target cell’s peptide chain elongation factor Ⅱ) Target cell death), the constructed gene recombinant immunotoxins will be able to specifically kill a target cell melanoma cells. In this experiment, PCR techniques were used to obtain α-MSH and PE40 (Pseudomonas exotoxin-deficient cells bound to functional regions to avoid immunotoxin