论文部分内容阅读
目的:观察前痛宁方对慢性非细菌性前列腺炎(CAP)模型大鼠前列腺组织细胞因子TNF-α、IL-8、IL-10及β-EP的影响。方法:将40只SD雄性大鼠随机分成空白组、模型组、盐酸坦洛新组和前痛宁方组。对除空白组外的其余3组大鼠行去势手术后于背部皮下注射苯甲酸雌二醇法制作CAP模型,各组分别给予相对应的药物干预。检测比较各组前列腺组织TNF-α、IL-8、IL-10及β-EP的含量。结果:模型组大鼠前列腺组织TNF-α、IL-8含量较空白组明显升高(P<0.01),β-EP含量较空白组明显降低(P<0.01);盐酸坦洛新组β-EP含量较模型组明显升高(P<0.01);前痛宁方组TNF-α、IL-8含量均明显低于模型组和盐酸坦洛新组(P<0.01),IL-10、β-EP含量明显高于模型组和盐酸坦洛新组(P<0.01,P<0.05)。结论:多种细胞因子参与了CAP的发病过程;中药前痛宁方可能通过降低前列腺组织内促炎性因子TNF-α、IL-8的水平,升高抗炎性因子IL-10及β-EP的水平,从而减轻前列腺组织局部的炎症反应,缓解疼痛,促进前列腺组织的修复。
Objective: To observe the effect of QianTuNingFang on cytokine TNF-α, IL-8, IL-10 and β-EP in prostatic tissue in chronic nonbacterial prostatitis (CAP) model rats. Methods: 40 SD male rats were randomly divided into blank group, model group, tamsulosin hydrochloride group and Qiantongningfang group. The remaining 3 groups of rats except for the blank group underwent ovariectomy, and subcutaneous injection of estradiol benzoate in the back produced CAP model. Corresponding drug intervention was given in each group. The contents of TNF-α, IL-8, IL-10 and β-EP in prostatic tissue were detected and compared. Results: The levels of TNF-α and IL-8 in the model group were significantly higher than those in the blank group (P <0.01), while the content of β-EP was significantly lower in the model group than in the blank group (P <0.01) (P <0.01). The contents of TNF-α and IL-8 in Qiantuoningfang group were significantly lower than those in model group and tamsulosin hydrochloride group (P <0.01) -EP content was significantly higher than the model group and tamsulosin hydrochloride group (P <0.01, P <0.05). CONCLUSIONS: A variety of cytokines are involved in the pathogenesis of CAP. Chinese medicine Qiantongning Prescription may increase the levels of anti-inflammatory cytokines IL-10 and β-EP by decreasing the levels of pro-inflammatory cytokines TNF-α and IL-8 Level, thereby reducing the local inflammation of prostate tissue response, relieve pain, promote prostate tissue repair.