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以乙酸、草酸、盐酸、硫酸等单酸、二酸、三酸混和及纤维素酶降解植物纤维素的正交试验得出的最佳工艺条件为基础 ,进一步研究出一种原料连续添加和酸酶循环利用法降解植物纤维素新技术 (CACU法 )。该技术的整个过程只需要 12步操作 ,5或 6套设备 ,包括 3~ 4套反应釜和 2套贮罐。在常压、温度 10 0℃、反应 15 h的条件下 ,以 CH3COOH,H Cl和纤维素酶为反应剂 ,按照该工艺技术能使纤维素转化成葡萄糖的转化率达 95 .3 4% ,反应终液中的葡萄糖浓度达 3 .2 1%。与常规技术相比 ,该新技术只需 2 5 %的醋酸和 5 0 %的盐酸量 ,可以较大幅度地降低纤维素转化成葡萄糖的成本。因此 ,该技术具有条件要求低、工艺简单、成本低和周期短等特点 ,是一种值得进一步开发和推广的技术。
Based on the optimum technological conditions obtained from the orthogonal experiment of single acid, diacid, triacid and cellulose degradation of plant cellulose by acetic acid, oxalic acid, hydrochloric acid, sulfuric acid and so on, we further studied a method of continuous addition of raw materials and acid Enzymatic recycling of plant cellulose degradation of new technology (CACU method). The entire process of this technology requires only 12 steps, 5 or 6 sets of equipment, including 3 to 4 sets of reactors and 2 storage tanks. Under the conditions of atmospheric pressure, temperature 10 0 ℃ and reaction for 15 h, CH3COOH, HCl and cellulase were used as reactants. According to this technology, the conversion of cellulose to glucose reached 95.34% The final glucose concentration in the reaction solution reached 3.2%. Compared with the conventional technology, the new technology requires only 25% acetic acid and 50% hydrochloric acid, which can greatly reduce the cost of converting cellulose into glucose. Therefore, the technology has the characteristics of low requirements, simple process, low cost and short cycle, which is a technology worthy of further development and promotion.