论文部分内容阅读
利用多重PCR结合DHPLC技术首次建立转基因玉米高通量快速检测方法。根据公布的转基因玉米外源基因序列设计并合成适合多重PCR的引物序列,以转基因玉米为模板,通过优化多重PCR和DHPLC的反应条件,建立一套同时快速筛选检测玉米中多种转基因成分的7重PCR-DHPLC检测方法。该方法的检测灵敏度为0.195 ng/μL,质粒检测灵敏度为1×103拷贝/μL。
Using multiplex PCR combined with DHPLC technology to establish a high-throughput rapid detection method for transgenic corn. According to the published sequence of exogenous gene of transgenic maize, primer sequences suitable for multiplex PCR were designed and synthesized. Using the transgenic maize as template, a set of simultaneous and rapid screening methods for detecting multiple transgenic components in maize was established by optimizing the reaction conditions of multiplex PCR and DHPLC Heavy PCR-DHPLC detection method. The detection sensitivity of the method was 0.195 ng / μL, and the sensitivity of plasmid detection was 1 × 103 copies / μL.