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目的用鸡建立迟发性神经病的动物模型,观察其病理改变。方法分为甲胺磷造模组、磷酸三甲苯酯造模组和对照组,甲胺磷造模组经口给予甲胺磷10 mg/(kg.d),连续给药14 d;磷酸三甲苯酯造模组经口给予磷酸三甲苯酯原油1 ml/(kg.d),连续给药7 d;对照组给予生理盐水,观察中毒表现和病理学变化。结果甲胺磷造模组的动物,经口小剂量多次累计染毒至5倍LD50以上,60%可出现迟发性神经病的症状;磷酸三甲苯酯造模组的动物,染毒后7~10 d全部出现典型的迟发性神经病症状;两组有症状动物的脑、脊髓及坐骨神经均出现脱髓鞘等明显的神经损害。结论磷酸三甲苯酯比甲胺磷更易建立实验性鸡迟发性神经病的动物模型。
Objective To establish an animal model of delayed neuropathy with chicken and observe its pathological changes. Methods: Methamidophos model group, tricresyl phosphate model group and control group, methamidophos model group were given methamidophos 10 mg / (kg.d), continuous administration of 14 d; tris The cresyl phosphate model group was orally administered with 1 ml / (kg · d) of tricresyl phosphate crude oil for 7 days. The control group was given normal saline, and the poisoning and pathological changes were observed. Results The animals in the methamidophos group were exposed to LD50 more than 5 times after 60 days of oral low dose. The symptoms of delayed neuropathy were observed in 60% of the animals. The animals in the model group with tricresyl phosphate ~ 10 d all appeared typical symptoms of delayed neuropathy; two groups of symptomatic animals in the brain, spinal cord and sciatic nerve demyelination and other obvious neurological damage. Conclusion Tricresyl phosphate is more likely to establish an animal model of experimental delayed neuropathy in children than methamidophos.