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目的探讨三氯异氰尿酸消毒剂对酶联免疫法(ELISA)测定造成的假阳性影响,为免疫实验室合理使用消毒剂和排除假阳性提供依据。方法将常规三氯异氰尿酸消毒剂消毒浓度按1∶50、1∶100、1∶200、1∶400、1∶800、1∶1 600配成不同的浓度,采用夹心ELISA(以乙肝表面抗原试剂进行试验),以自带的阴性血清和阳性血清(1 IU/ml)作为质控进行试验,至加入显色剂后,实验组加入各浓度的三氯异氰尿酸消毒剂,常规显色后在波长450 nm和加终止液后按试剂要求波长进行测定。结果各浓度的三氯异氰尿酸消毒剂可使ELISA各孔的OD值升高,达阳性的判断标准,而没加终止液前的显色不会随显色时间的变化而变化。结论三氯异氰尿酸消毒剂可致ELISA检测结果出现假阳性,免疫实验室在使用该消毒剂时要避免污染实验操作及密切观察加显色剂后的颜色变化,以便及时发现消毒剂的假阳性影响,保证检验结果的可靠性。
Objective To explore the influence of trichloroisocyanuric acid disinfectant on the false positives caused by enzyme-linked immunosorbent assay (ELISA), and provide the basis for the rational use of disinfectants and the elimination of false positives in immune laboratories. Methods The disinfection concentration of conventional trichloroisocyanuric acid disinfectant was dubbed into different concentrations according to 1:50, 1: 100, 1: 200, 1: 400, 1: 800 and 1: Antigen reagent to test), with negative serum and positive serum (1IU / ml) as a quality control test, to join the reagent, the experimental group was added to the concentration of TCA disinfectant, the conventional significant After the wavelength of 450 nm and plus stop solution after the reagent required wavelength determination. Results Trichloroisocyanuric acid disinfectants at various concentrations increased the OD values in each well of the ELISA to a positive standard of judgment without any change in color development with no change in colorimetric time. Conclusion Trichloroisocyanuric acid disinfectant can cause false positive ELISA test results, immunological laboratories in the use of the disinfectant to avoid contamination of the experimental operation and close observation of the color change after the addition of the color reagent in order to promptly find the disinfectant fake Positive effect, to ensure the reliability of test results.