论文部分内容阅读
目的 比较不同乙型肝炎病毒 (HBV)株在体内及转染细胞中复制特性是否相符。方法 以核酸杂交定量和聚合酶链反应 酶联免疫吸附试验 (PCR ELISA)测定 5例孕妇血清中HBVDNA含量 ,并测定乙型肝炎表面抗原 (HBsAg)和乙型肝炎e抗原 (HBeAg)含量。分别克隆血清中的HBV基因组转染细胞 ,检测培养上清液中HBsAg和HBeAg表达水平 ,并以Southern印迹及核酸杂交检测转染细胞内、外HBVDNA复制水平。结果 转染细胞内、外HBVDNA复制水平与相应血清HBVDNA量呈正相关趋势。转染细胞表达的病毒抗原水平与相应血清中病毒抗原含量也呈正相关趋势。结论 感染者血清中HBVDNA和病毒抗原的含量与毒株在细胞中复制和抗原表达水平有相符趋势。HBV不同毒株在转染细胞中的复制可基本反映体内毒株的复制特性。
Objective To compare the replication characteristics of different hepatitis B virus (HBV) strains in vivo and in transfected cells. Methods The contents of HBVDNA in serum of 5 pregnant women were detected by quantitative PCR and polymerase chain reaction (ELISA), and the contents of HBsAg and HBeAg were determined. The HBV genomes were cloned respectively and the expression of HBsAg and HBeAg in the culture supernatant was detected. The level of HBVDNA replication in transfected cells was detected by Southern blotting and nucleic acid hybridization. Results The level of HBVDNA replication in transfected cells was positively correlated with the level of serum HBVDNA. The level of virus antigen expressed in the transfected cells also showed a positive correlation with the level of virus antigen in the corresponding serum. Conclusion The contents of HBVDNA and virus antigens in the serum of infected persons are consistent with the replication and antigen expression level of the virus in the infected cells. The replication of different HBV strains in the transfected cells can basically reflect the replication characteristics of the in vivo strains.