目的：观察缺血性急性肾功能衰竭时肾组织细胞内三磷酸肌醇（ＩＰ３）的含量变化。方法：用新西兰大白兔制作缺血性急性肾功能衰竭模型。观察缺血性急性肾功能衰竭组（简称肾衰组）肾组织细胞内胞浆游离钙（［Ｃａ２＋］ｉ）和第二信使———三磷酸肌醇的浓度变化。结果：肾衰组［Ｃａ２＋］ｉ为４１２１±４７３ｎｍｏｌ／Ｌ，ＩＰ３为７７６０±６８０ｐｃｍ，较之对照组的［Ｃａ２＋］ｉ（１０６１±１５５ｎｍｏｌ／Ｌ），ＩＰ３（２９７９±５４３ｐｃｍ）含量明显增高（均为Ｐ＜００１）。结论：缺血性急性肾功能衰竭时肾组织细胞内钙超负荷与细胞内ＩＰ３含量升高有关 Objective: To observe the change of inositol 1,4,5 - trisphosphate (IP3) content in renal tissue of rats with acute ischemic acute renal failure. Methods: New Zealand white rabbits were used to make ischemic acute renal failure model. To observe the change of cytosolic free calcium ([Ca2 +] i) and the second messenger-inositol 1,4,5-trisphosphate in renal tissue of ischemic acute renal failure group (renal failure group). Results: Compared with [Ca2 +] i (106.1 ± 15.5nmol / L) in control group, [Ca2 +] i in renal failure group was 412.1 ± 47.3nmol / L and IP3 was 776.0 ± 68.0pcm , IP3 (2979 ± 543pcm) were significantly increased (all P <001). Conclusion: Intracellular calcium overload in renal tissue is related to the increase of intracellular IP3 in ischemic acute renal failure