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本文用Ames试验方法和微核试验方法,对厨房煎炸油烟物质的致突变性进行了研究。结果Ames试验阴性,而微核试验则呈现阳性结果。多数学者认为Ames试验与微核试验有良好的相关性,而且Ames试验较微核试验敏感。作者认为本研究出现上述结果的原因,可能是由油烟物质具有较强的刺激性所致。作者在实验中发现,油烟物质具有较强的抑菌作用,当样品稀释至100倍时,抑菌作用才消失,而这一浓度可能不足以引起细菌的回变。这就是说,油烟物质的致突变浓度可能高于对细菌本身的抑制浓度,结果导致Ames试验呈现阴性。
In this paper, Ames test method and the micronucleus test method, the mutagenicity of fried soot in the kitchen was studied. Results Ames test negative, while the micronucleus test showed positive results. Most scholars believe that Ames test and micronucleus test have good correlation, and Ames test is more sensitive than micronucleus test. The author believes that the reason for the above results in this study may be caused by the strong irritation of fume substances. The authors found in the experiment, the fume substance has a strong antibacterial effect, when the sample diluted to 100 times, the antibacterial effect disappeared, and this concentration may not be sufficient to cause bacteria to change back. This means that the mutagenic concentration of the fuma substance may be higher than that of the bacterium itself, resulting in the Ames test being negative.