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采用高效液相色谱法,以电化学检测测定黄芩苷的血浆药物浓度.流动相0.1mol·L-1磷酸二氢钾:乙酸:四氢呋喃(1000:100:190),色谱柱ERC-ODS-1161(6mm×100mm),检测电压+650 mv,柱温55℃,流速08 ml·min-1.结果:该方法的最小检测浓度为50 ng·mL-1,平均回收率93.6%,重现性好.
High-performance liquid chromatography was used to determine the plasma concentration of baicalin by electrochemical detection. Mobile phase 0.1mol·L-1 potassium dihydrogen phosphate: acetic acid: tetrahydrofuran (1000: 100: 190), column ERC-ODS-1161 (6mm × 100mm), detection voltage +650 mv, column temperature 55 ℃, ml · min-1. Results: The minimum detectable concentration of this method was 50 ng · mL-1 with an average recovery of 93.6%. The reproducibility was good.