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采用高效液相色谱法，以电化学检测测定黄芩苷的血浆药物浓度．流动相０．１ｍｏｌ·Ｌ－１磷酸二氢钾：乙酸：四氢呋喃（１０００：１００：１９０），色谱柱ＥＲＣ－ＯＤＳ－１１６１（６ｍｍ×１００ｍｍ），检测电压＋６５０ ｍｖ，柱温５５℃，流速０８ ｍｌ·ｍｉｎ－１．结果：该方法的最小检测浓度为５０ ｎｇ·ｍＬ－１，平均回收率９３．６％，重现性好．
High-performance liquid chromatography was used to determine the plasma concentration of baicalin by electrochemical detection. Mobile phase 0.1mol·L-1 potassium dihydrogen phosphate: acetic acid: tetrahydrofuran (1000: 100: 190), column ERC-ODS-1161 (6mm × 100mm), detection voltage +650 mv, column temperature 55 ℃, ml · min-1. Results: The minimum detectable concentration of this method was 50 ng · mL-1 with an average recovery of 93.6%. The reproducibility was good.