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用液体闪烁计数法测定离体灌注缺氧鼠心线粒体内~(45)Ca的放射性强度(cpm),比较ATPMgCl_2,ATP-MS~(2+)和ATP对离体灌注缺氧鼠心线粒体内钙离子浓度的影响,结果表明,ATPMgCl_2,ATP-Mg~(2+)和ATP组(45)~Ca的cpm在离体灌注缺氧20min以内显著低于对照组(P<0.01);ATP-MgCl_2,和ATP-Mg~(2+)组(45)~Ca的cpm又显著低于ATP组(P<0.01),而ATP-MgCl_2和ATP-Mg~(2+)组间无差异(P>0.05)。提示外源性ATP能够抑制离体灌注缺氧鼠心线粒体内钙离子沉积,以ATP-MgCl_2或ATP-Mg~(2+)形式静脉给药时效果更佳,对实验性缺氧心肌细胞有保护作用。
The radioactive intensity (cpm) of ~(45)Ca in the mitochondria of isolated hypoperfused rat hearts was measured by liquid scintillation counting method. The effects of ATPMgCl2, ATP-MS~(2+) and ATP on the mitochondrial anoxia in isolated mouse hearts were compared. The effects of calcium ion concentration showed that the cpm of ATFMgCl2, ATP-Mg~(2+) and ATP groups (45)~Ca were significantly lower than those of the control group within 20 min of hypoxia (P<0.01); The cpm of (45)~Ca in the MgCl 2 and ATP-Mg 2+ groups was significantly lower than that in the ATP group (P<0.01), but there was no difference between the ATP-MgCl 2 and ATP-Mg 2+ groups (P >0.05). It is suggested that exogenous ATP can inhibit the mitochondrial calcium ion deposition in hypoxic rat hearts after perfusion, and the effect is better when administered intravenously in the form of ATP-MgCl 2 or ATP-Mg 2+ , and it has an effect on experimental hypoxic cardiomyocytes. Protective effects.