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饲料植物中的淀粉,常用伯川氏法测糖值计算淀粉含量。该法步骤繁杂,费时。蒽酮比色法测糖的特点是简易快速,准确性高,比伯川氏法可提高工效4—5倍。 1.标准曲线绘制:吸取0.1g/葡萄糖标准液10ml,注入100ml容量瓶中,并定容至刻度。吸取该液1,2,3,4,5ml分别注入5个50ml的三角瓶中,各加入蒸馏水至5ml,浓度分别为100、200、300、400、500μg/ml(测空白)。小心加入蒽酮试剂(制
Starch in feed plants, often used to determine the sugar content of Starch’s content. The procedure is complicated and time-consuming. Anthrone colorimetric method for measuring sugar is simple and fast, high accuracy, Bachelet’s method can improve the efficiency of 4-5 times. 1. Standard curve drawing: Pipette 0.1g / glucose standard solution 10ml, into 100ml volumetric flask, and set the volume to the mark. Pipette 1, 2, 3, 4, 5ml were injected into five 50ml conical flask, each adding distilled water to 5ml, the concentrations were 100,200,300,400,500 g / ml (measured blank). Carefully add anthrone reagent (system