Host-pathogen interactions are highly complex,involving large dynamic changes in gene expression during infection.These interactions are fundamental to understanding anti-infection immunity of hosts,as well as the pathogenesis of pathogens.For bacterial pathogens interacting with animal hosts,time-resolved dual RNA-seq of infected tissue is difficult to perform due to low pathogen load in infected tissue.In this study,an acute infection model of Larimichthys crocea infected by Pseudomonas plecoglossicida was established.The spleens of infected fish exhibited typical symptoms,with a maximum bacterial load at two days post-injection (dpi).Time-resolved dual RNA-seq of infected spleens was successfully applied to study hostpathogen interactions between L.crocea and P.plecoglossicida.The spleens of infected L.crocea were subjected to dual RNA-seq,and transcriptome data were compared with those of noninfected spleens or in vitro cultured bacteria.Results showed that pathogen-host interactions were highly dynamically regulated,with corresponding fluctuations in host and pathogen transcriptomes during infection.The expression levels of many immunogenes involved in cytokine-cytokine receptor,Toll-like receptor signaling,and other immunerelated pathways were significantly up-regulated during the infection period.Furthermore,metabolic processes and the use of oxygen in L.crocea were strongly affected by P.plecoglossicida infection.The WGCNA results showed that the metabolic process was strongly related to the entire immune process.For P.plecoglossicida,the expression levels of motility-related genes and flagellum assemblyrelated genes were significantly up-regulated.The results of this study may help to elucidate the interactions between L.crocea and P.plecoglossicida.