论文部分内容阅读
目的:观察丹酚酸胶囊的抗心肌缺血作用.方法:56只体重为(12±2)kg的健康Beagle犬,雌雄兼用,随机分为假手术组,模型对照组、高(100 mg/kg)、中(50 mg/kg)、低(25 mg/kg)剂量的丹酚酸胶囊组,丹参片组(275 mg/kg),地奥心血康胶囊组(100 mg/kg).采用结扎冠状动脉前降支法致Beagle犬急性心肌缺血,经十二指肠给药后,用心外膜电图标测法计算心肌缺血程度,硝基四氮唑蓝(NBT)组织化学染色法测定心肌梗死面积,检测血清中磷酸肌酸激酶(CK)和乳酸脱氢酶(LDH),观察丹酚酸胶囊对心肌缺血损伤的保护作用.结果:丹酚酸胶裳可明显降低心肌缺血程度(∑-ST),减少心肌缺血范围(N-ST),缩小心肌梗死面积(MIS),并能显著降低血清CK和LDH活力,明显减轻心肌细胞损伤程度.结论:丹酚酸胶囊对急性心肌缺血犬具有明显的保护作用.“,”AIM: To observe the effect of salvian-olic acid capsule on acute myocardial ischemia in Beagle dogs. METHODS: 56 Beagle dogs weighting (12 ± 2) kg were randomly divided into 7 groups, which including sham operation group, model control group, salvianolic acid capsule group (25, 50, 100 mg/kg), salvia tablets group(275 mg/kg), Diaoxinxuekang capsule group (100 mg/kg). The acute myocardial ischemia model in Beagle dogs were established by ligating anterior descending coronary artery after duodenal administration . The degree of myocardial ischemia was detected by epicardial electrogram mapping and the area of myocardial infarction was determined by NBT immu-nohistochemistry. The levels of serum CK and LDH were detected. The heart muscle ischemia protection of salvianolic acid capsule were observed. RESULTS: The salvianolic acid capsule could degrade the extent of heart muscle ischemia (∑-ST), decrease the range of heart muscle ischemia (N-ST), deflate the myocardial infarction size (MIS), relieve the levels of serum CK and LDH significantly, and relieve the injury degree of cadiocyte. CONCLUSION: Salvianolic acid capsule has remarkable protective effect on acute myocardial ischemia in Beagle dogs.