本文报导了青霉素在氢氧化钠介质中水解为具有还原性的青霉素噻唑酸后,在沸水加热条件下,于0.24mol/l硫酸介质中,利用磷钼酸铵被还原为钼兰的水相显色反应;以分光光度法来测定青霉素的含量.兰色络合物色泽稳定1小时以上,方法灵敏、准确、简便. This paper reports the hydrolysis of penicillin in sodium hydroxide medium to a reducing penicillin thiazole acid, in boiling water heating conditions, in 0.24mol / l sulfuric acid medium, the use of ammonium phosphomolybdate was reduced to molybdenum blue water phase was Color reaction to determine the content of penicillin spectrophotometry blue complex color stability for more than 1 hour, the method is sensitive, accurate and easy.