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腺苷酸环化酶是位于细胞膜上的一种复杂的酶系统,它通过鸟苷酸调节蛋白与膜表面激素受体相偶联,催化细胞内ATP生成cAMP,介导外部信息的跨膜传递。作者以1968年Krishna等建立的方法为基础,综合有关改进方法并加以简化。断头处死大鼠,迅速取出所需组织(脑、肝、肾、肌肉等)于冰浴中制成匀浆,腺苷酸环化酶活力测定反应液的总体积为200μl,其中加入50mM Tris-HCl,pH7.5,8mM茶碱,5mM
Adenylate cyclase is a complex enzyme system located on the cell membrane. It is coupled to membrane-surface hormone receptors by guanylin-regulating proteins and catalyzes the intracellular ATP production of cAMP, which mediates the transmembrane transmission of external information . Based on the method established by Krishna et al. In 1968, the author synthesizes the related improvement methods and simplifies them. The rats were decapitated and the desired tissues (brain, liver, kidney, muscle, etc.) were quickly removed and homogenized in an ice bath. The total volume of the adenylate cyclase activity assay reaction solution was 200 μl, to which 50 mM Tris HCl, pH 7.5, 8 mM theophylline, 5 mM