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目的 通过动物试验评价土贝母皂甙 (tubeimoside ,Tu)治疗单纯疱疹病毒性角膜炎 (herpessimplexkeratitis ,HSK)的效果及点眼刺激性 ,并确定其点眼浓度值。方法 根据药物的眼刺激性选用 5、2、0 .8、8× 10 -2 、8× 10 -3、8× 10 -4 g·L-1Tu点眼 ,确定 0 .4、0 .2、0 .1g·L-1评价药物疗效。在地鼠肾细胞BHK 2 1上进行单纯疱疹病毒Ⅰ型(HSV 1型 )SM4 4毒株复苏 ,制作兔HSK模型。采用裂隙灯显微镜、扫描电镜和透射电镜观察眼部病变的动态变化评价Tu疗效。结果 5、2 g·L-1Tu有中度刺激性 ,0 .8g·L-1Tu有轻度刺激性。 8× 10 -2 、8× 10 -3、8× 10 -4 g·L-13组Tu无任何眼刺激性。 0 .4g·L-1Tu有轻度刺激性 ,0 .1、0 .2 g·L-1Tu无刺激性。 0 .4、0 .2、0 .1g·L-13组Tu均具有一定疗效。结论 Tu点眼剂的浓度与刺激性呈正相关 ,点眼刺激性从轻度到无的界值浓度为 0 .4、0 .2、0 .1g·L-1。治疗实验性HSK有一定的疗效 ,但尚不及无环鸟苷
OBJECTIVE: To evaluate the efficacy and irritation of herpes simplex keratitis (HSK) by tubeimoside (Tu) in animal experiments and to determine the ophthalmic concentration. Methods According to the ophthalmic irritation of the drugs, 5,2,0. 8, 8 × 10 -2, 8 × 10 -3 and 8 × 10 -4 g · L -1 Tu were selected for eye irritation. The values of 0.4, .1g · L-1 evaluation of drug efficacy. Herpes simplex virus type 1 (HSV type 1) SM4 4 strain was resuscitated on hamster kidney cell BHK 2 1 to prepare a rabbit HSK model. Slit lamp microscopy, scanning electron microscopy and transmission electron microscopy were used to observe the dynamic changes of ocular lesions to evaluate the efficacy of Tu. Results 5,2 g · L-1Tu moderate irritant, 0. 8g · L-1Tu mild irritant. 8 × 10 -2, 8 × 10 -3, 8 × 10 -4 g · L-13 Tu without any eye irritation. 0 .4g · L-1Tu mild irritant, 0 .1,0 .2 g · L-1Tu non-irritating. 0 .4,0 .2,0 .1 g · L-13 group Tu have a certain effect. Conclusions The concentration of Tu ophthalmic solution is positively correlated with the stimulus. The threshold irritant concentration is 0. 4,0. 2,0. 1g · L-1 from mild to no. Treatment of experimental HSK have a certain effect, but not as acyclovir