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目的 研究肝硬化患者血浆能否刺激血管内皮细胞产生一氧化氮。方法 分别用正常人血浆和肝硬化患者血浆刺激人脐静脉血管内皮细胞,以硝酸还原酶法测定细胞培养上清NO浓度,逆转录-聚合酶链反应(RT-PCR)测定血管内皮细胞NO(eNOs)mRNA水平。结果肝硬化患者血浆组NO浓度显著高于正常人血浆组(P≤0.01);门静脉高压症患者血浆处理组eNOS mR-NA水平较正常人血浆组增高,且有时间依赖性,正常人血浆处理样品的各个时间点其RNA水平没有明显差异。结论 肝硬化患者血浆本身可刺激血管内皮细胞产生NO。
Objective To study whether plasma of patients with cirrhosis can stimulate the production of nitric oxide by vascular endothelial cells. Methods The human umbilical vein endothelial cells were stimulated with plasma from patients with normal plasma and cirrhosis respectively. The concentrations of NO in the culture supernatant were determined by nitrate reductase method. The levels of NO (superscript -) were determined by reverse transcription polymerase chain reaction (RT-PCR) eNOs) mRNA levels. Results The plasma concentration of NO in patients with cirrhosis was significantly higher than that in normal people (P≤0.01). The plasma levels of eNOS mR-NA in patients with portal hypertension were significantly higher than those in normal people There was no significant difference in RNA level between samples at each time point. Conclusion The plasma of patients with cirrhosis can stimulate NO production in vascular endothelial cells.