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为研发净化水产养殖废水的菌藻固定化体,以蛋白核小球藻(Chlorella pyrenoidosa Chick)、光合细菌(Photosynthetic bacteria,PSB)和海藻酸钠、CaCl_2等为试验材料,采用正交法等方法,研究了菌藻固定球的制备方法与条件,比较分析了固定化菌藻球对养殖废水的净化去除效果。结果表明,包埋蛋白小球藻和光合细菌的最适条件为2%海藻酸钠与6%氯化钙交联24 h。养殖废水中,藻菌固定球在1~4 d对PO_4~(3-)-P去除率显著高于蛋白核小球藻和光合细菌(P<0.05),在5~8 d对NH_4~+-N去除率也显著高于后两者。固定化菌藻球在24℃对磷和氮去除率最高分别可达84%和95%。本研究表明,合理的固定和使用菌藻结合体可显著提高对养殖废水的净化效率,使用藻菌固定化球净化养殖废水比单独使用菌类或藻类效果更好。
In order to develop a strain of immobilized algae that purifies aquaculture wastewater, Chlorella pyrenoidosa Chick, Photosynthetic bacteria (PSB) and sodium alginate, CaCl 2 were used as experimental materials. Orthogonal methods , The preparation methods and conditions of the bacteria-immobilized spherulites were studied, and the effects of the immobilized bacteria algae spheres on the purification and removal of aquaculture wastewater were analyzed. The results showed that the optimal conditions for embedding Chlorella vulgaris and photosynthetic bacteria were 2% sodium alginate and 6% calcium chloride for 24 h. In aquaculture wastewater, the removal rates of PO_4 ~ (3 -) - P from 1 to 4 days were significantly higher than those from C. alga and C. aeruginosa (P <0.05) -N removal rate is also significantly higher than the latter two. Immobilized algae spheres at 24 ℃ for phosphorus and nitrogen removal rates up to 84% and 95%, respectively. This study shows that the reasonable immobilization and use of bacteria and algae combination can significantly improve the efficiency of the purification of aquaculture wastewater, the use of algae immobilized ball purification of aquaculture wastewater than the use of fungi alone or algae better.