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目的通过糖代谢紊乱状态患者血清基质细胞衍生因子-1α(SDF-1α)与循环内皮祖细胞(EPCs)的相关性研究,阐明糖代谢紊乱SDF-1对EPCs的影响。方法采集60名正常成人、78例IGT、71例无血管并发症T2DM患者、60例血管并发症T2DM患者的外周血,以密度梯度离心法获得单个核细胞分离鉴定EPCs,分别测定EPCs的数量、迁移、黏附、增殖、体外血管生成能力,ELISA检测SDF-1α。分析受试者SDF-1、EPCs的差异,观察各组SDF-1α与EPCs的数量、迁移、黏附、增殖、体外血管生成能力的相关性。结果正常、IGT、无血管并发症T2DM、血管并发症T2DM组SDF-1为[(6.52±1.04)、(6.23±1.02)、(5.98±0.97)、(5.39±0.85)]μg/L,EPCs(数量、迁移、黏附、增殖及体外血管生成能力)为[(53.7±6.7)、(35.17±4.39)、(25.98±4.39)、(17.24±2.15),(9.7±2.2)、(6.27±1.42)、(4.11±0.93)、(2.35±0.53),(22.9±4.3)、(17.36±3.26)、(12.36±2.32)、(7.73±1.43),(0.62±0.04)、(0.51±0.03)、(0.38±0.03)、(0.29±0.02)]cell/200及[(11.3±1.8)、(8.82±1.32)、(5.69±0.91)、(4.10±0.65)]tubules/200(P<0.01)。相关分析结果表明,IGT组SDF-1α只与EPCs的数量呈正相关(P<0.05),无血管并发症T2DM、血管并发症T2DM组中SDF-1α与EPCs中的数量、迁移、黏附、增殖、体外血管生成能力均呈正相关(P<0.05或P<0.01)。结论糖代谢紊乱对EPCs的影响是通过SDF-1α产生,这对开辟新的T2DM防治途经找到了新的线索。
OBJECTIVE: To investigate the relationship between SDF-1α and EPCs in patients with glycometabolism disorders (SDF-1α) and EPCs (EPCs). Methods Peripheral blood samples from 60 normal adults, 78 IGT patients, 71 T2DM patients without vascular complications and 60 T2DM patients with vascular complications were collected. EPCs were isolated and identified by density gradient centrifugation. The numbers of EPCs, Migration, adhesion, proliferation, in vitro angiogenesis ability, ELISA detection of SDF-1α. The differences of SDF-1 and EPCs between the two groups were analyzed. The correlation between SDF-1α and the number of EPCs, migration, adhesion, proliferation and angiogenesis in vitro were observed. The results of normal, IGT, T2DM without vascular complications and T2DM of vascular complications were (6.52 ± 1.04), (6.23 ± 1.02), (5.98 ± 0.97), (5.39 ± 0.85) μg / L, (53.7 ± 6.7), (35.17 ± 4.39), (25.98 ± 4.39), (17.24 ± 2.15), (9.7 ± 2.2), (6.27 ± 1.42 (4.11 ± 0.93), (2.35 ± 0.53), (22.9 ± 4.3), (17.36 ± 3.26), (12.36 ± 2.32), (7.73 ± 1.43), (0.62 ± 0.04), (0.51 ± 0.03) (0.38 ± 0.03), (0.29 ± 0.02)] cells / 200 and [(11.3 ± 1.8), (8.82 ± 1.32), (5.69 ± 0.91), (4.10 ± 0.65)] tubules / 200, respectively. Correlation analysis showed that there was a positive correlation between SDF-1α and the number of EPCs in IGT group (P <0.05), the number of SDF-1α and EPCs in T2DM without vascular complications, vascular adhesion, proliferation, In vitro angiogenic capacity was positively correlated (P <0.05 or P <0.01). Conclusion The effect of glucose metabolism disorders on EPCs is generated through SDF-1α, which has found new clues to open up new prevention and treatment of T2DM.