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近几年来,国内外对染色体制片和G显带方法上取得了不少进展。本室在借鉴其它方法的基础上建立了一种较稳定的制备染色体和G显带方法,特别在取绒毛量很少时也可制成供染色体分析的标本。 1.将标本放入0.9%的生理盐水中,在镜下挑出绒毛。 2.将绒毛放入1%柠檬酸钠溶液3ml中(每ml含有0.5μg秋水仙胺),在37℃下低渗30分钟。 3.加入3:1甲醇-冰乙酸固定液1ml,混匀待分层后立即吸出上清液。再加入8:1甲醇-冰乙酸固
In recent years, a lot of progress has been made at home and abroad on methods of chromosome production and G banding. Based on other methods, this laboratory has established a more stable preparation method for chromosomes and G banding, especially when the amount of villus is small, it can also be used as a specimen for chromosome analysis. 1. Place the specimen in 0.9% physiological saline and pick out the villi under the microscope. 2. The fluff was placed in 3 ml of 1% sodium citrate solution (containing 0.5 [mu]g of colcemid per ml) and hypotonic for 30 minutes at 37[deg.]C. 3. Add 1 ml of a 3:1 methanol-glacial acetic acid fixative and mix until the supernatant is aspirated immediately after delamination. Then add 8:1 methanol-acetic acid