Serial nuclear transfer improves the development of interspecies reconstructed giant panda (Aluropod

来源 :科学通报 | 被引量 : 0次 | 上传用户:zj2008263
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Interspecies somatic nuclear transfer (NT) may provide a new approach for preservation of the endangered rare species. Previous interspecies cloning studies have shown that a nucleus from a quiescent somatic cell supports early development of reconstructed embryos in the ooplasm from another species. In this study, we transferred nonquiescent somatic cells from a giant panda into the perivitelline space of the enucleated rabbit oocytes. After electrofusion (at the rate of 71.6%) and electrical activation, 4.2% of the panda-rabbit reconstructed embryos developed to blastocyst in vitro. For improving the development rate of reconstructed embryos, we used serial NT in this study, I.e. Blastomeres from reconstructed morulae were transferred into the perivitelline space of the enucleated rabbit oocytes. The fusion rates in the groups of serial Ⅰ, serial Ⅱ and serial Ⅲ were 79.5%, 84.1% and 78.0%, respectively, having no difference with that of somatic group. And the blastocyst rates in serial NT groups were 19.4%, 13.5% and 10.3%, respectively, which are significantly higher than that in somatic NT group. These results indicate that the nuclei from nonquiescent somatic cells can support early development of reconstructed embryos and serial NT can improve the development rate of interspecies reconstructed embryos.
其他文献
树杪炊烟一缕斜,穿云攀柳到山家。葡萄架底新醅酒,翡翠枝头昨夜花。粳米煎香川上水,瓦壶煮碧雨前茶。大哥斟罢小姑劝,盏盏深情醉晚霞。
漏夕朦胧月。京城忆鼓轮。双眸浮丽影,千里念芳邻。耳畔呢喃在,云边沥胆珍。何当再聚首,藉我故年心。
晦冷天开气转阳,轻风初起意犹凉。不须雪化梅花放,可待冰消雁字长。 山麓白,柳梢黄。盼伊消息每临窗。寒封三尺江河瘦,唯有相思未冻僵。
Two different length fragments, RSF1 and RSF2 which contained the cis-acting sequences of root-spe- cific gene TobRB7, were isolated from tobacco genome. The ab
运用GIS和RS技术 ,以LANDSATTM为数据源 ,监测我国草地资源近 5a来的变化 ,并对变化进行分析。研究表明 ,我国草地面积总量增加约 1 3 2× 1 0 4hm2 ,其中高覆盖度与中覆盖度草地面积增多 ,但低覆盖度草地面积减少。我国西部的草地面积减少 ,山西 ,内蒙古 ,吉林 ,陕西 ,甘肃 ,青海 ,宁夏 ,新疆均有不同程度的减少。其中内蒙古 ,新疆的各类草地变化显著。草地的变化有
琼楼玉阁似行宫,面水环山势更雄。科甲繁荣遗旧梦,丹墀壮丽自秋风。三门尽显皇恩荡,一院难收昔日隆。经国诗文传百祀,赤心道义古今同。
The plasmid containing the promoter Act1, the coat protein (cp) gene of wheat yellow mosaic virus (WYMV) and the selectable bar gene, was delivered via particle
对四川紫色土区土壤养分径流和泥沙流失特征进行了研究,结果表明:土壤氮素主要随径流流失,主要土内径流和地下径流携带NO3-N所造成;土壤磷主要随泥沙流失,泥沙中的养分有明显
The characteristics of dry matter production before and after heading and the relationships between photosynthesis of flag leaves and dry matter accumulation in
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7