据有关资料记载,湖北省自1960年始发现旋毛虫病流行,近年来其检出阳性率有所增加。我们应用酶联免疫吸附试验(ELISA)进行了本病的血清学调查研究,现报道如下。 材料与方法 一、旋毛虫抗原 自制。选感染旋毛虫密度高的鲜猪肉数斤,分离纯瘦肉并剪碎。经胃蛋白酶人工消化法分离肌旋毛虫,多次反复漂洗后经超声隔水破碎 According to the relevant information, it is found that the prevalence of Trichinosis has been found in Hubei province since 1960, and the positive rate has been increased in recent years. We applied the enzyme-linked immunosorbent assay (ELISA) serological investigation of the disease, are reported below. Materials and methods First, Trichinella antigen self-made. Trichinella selected high density of fresh pork a few pounds, pure lean meat and cut. Gemcitabine artificial digestion separation of Trichinella spiralis, repeatedly rinsed by ultrasonic water after crushing