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目的:观察全反式维甲酸(ATRA)对兔颈动脉粥样硬化性病灶中内膜增生、MCP-1及TLR-4表达的影响,探讨其可能的抗炎机制。方法:新西兰雄性大白兔随机分为9组(n=6):对照组(A、B、C)、治疗组(A、B、C)、假手术组(A、B、C)。除假手术组外,其余两组给予高脂饮食2周后,对照组及治疗组给予颈动脉内膜空气干燥术损伤颈动脉内膜,假手术组分离暴露颈动脉但不损伤内膜,治疗组术前3天给予ATRA灌胃,直至处死。术后分别于7d、14d、28d处死。采取颈动脉标本,对血管粥样硬化病变进行形态学观察及测定,采用免疫组化法检测MCP-1及TLR-4表达水平。结果:从形态学观察及免疫组化检测看,对照组较假手术组内膜明显增生,MCP-1及TLR-4表达增多,治疗组内膜较对照组增生减轻,两种因子表达减少。结论:全反式维甲酸(ATRA)对兔颈动脉粥样硬化性病灶中的抗炎作用可能是通过抑制MCP-1及TLR-4等炎症因子的表达来发挥作用的。
Objective: To observe the effect of all-trans retinoic acid (ATRA) on the intimal hyperplasia, the expression of MCP-1 and TLR-4 in rabbit carotid atherosclerotic lesions and to explore its possible anti-inflammatory mechanism. Methods: New Zealand white rabbits were randomly divided into 9 groups (n = 6): control group (A, B, C), treatment group (A, B, C) and sham operation group (A, B, C). Except sham group, the other two groups were given high-fat diet for 2 weeks, and the control group and the treatment group were given carotid intima with air-drying to injure the carotid intima. The sham-operated group was exposed carotid artery without injury to the intima, Group ATRA intragastric administration 3 days before surgery. After operation, they were sacrificed at 7d, 14d and 28d respectively. Carotid artery specimens were used to observe the morphological changes of vascular atherosclerosis and to determine the expression of MCP-1 and TLR-4 by immunohistochemistry. Results: Morphological observation and immunohistochemistry showed that the intimal hyperplasia and the expression of MCP-1 and TLR-4 in the control group were significantly increased compared with the sham-operation group. The intimal hyperplasia in the treatment group was reduced and the expression of the two factors was decreased. CONCLUSION: All-trans retinoic acid (ATRA) may have an anti-inflammatory effect on carotid atherosclerotic lesions in rabbits by inhibiting the expression of inflammatory cytokines such as MCP-1 and TLR-4.