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【目的】定位大豆倒伏性相关形态性状的QTL为培育抗倒伏性高的品种提供依据。【方法】以美国大豆品种Charleston为母本,东北农业大学大豆品系东农594为父本及其F2:16—F2:18的重组自交系的147个株系为试验材料,164个SSR引物经亲本筛选后用于群体扩增,并构建遗传图谱。在三年两个地点对大豆的主茎节数、茎粗和茎秆重性状进行调查及QTL分析。【结果】共检测到16个主茎节数QTL,分别位于A1、B1、C2、D1a、D2、F、G、H和N连锁群上;检测到10个茎粗QTL,分别位于A1、B1、C2、D1a、E和G连锁群上;检测到15个茎秆重QTL,分别位于A1、A2、C2、D1a、D1b和G连锁群上。在得到的这些QTL中,2种算法都能检测到5个主茎节数QTL,解释表型变异范围为8.6%—27.0%;1个茎粗QTL,解释表型变异范围为9.0%—11.0%;6个茎秆重QTL,解释表型变异范围为6.0%—39.0%。在2年以上能被检测到3个主茎节数QTL,解释表型变异范围为8.0%—60.2%;2个茎秆重QTL,解释表型变异范围为10.0%—23.0%;2年以上未重复检测到茎粗QTL。【结论】通过比较定位的主茎节数、茎粗和茎秆重QTL,发现这些性状之间存在较大的遗传相关性。
【Objective】 QTLs for mapping the morphological traits of soybean lodging provide the basis for breeding varieties with high lodging resistance. 【Method】 A total of 147 SSR lines from Charleston, a soybean of Northeast Agricultural University, and F2: 16-F2: 18 F2 population as test material, 164 SSR primers After the parental screening for population amplification and construction of genetic map. In three years and two locations on the main stems of soybean number, stem diameter and culm weight traits were investigated and QTL analysis. 【Result】 A total of 16 QTLs for main stems were detected and located on A1, B1, C2, D1a, D2, F, G, H and N linkage groups respectively. Ten QTLs were detected in A1, B1 , C2, D1a, E and G, respectively. Fifteen QTLs for stem cultrures were detected in the linkage groups A1, A2, C2, D1a, D1b and G, respectively. Among the obtained QTLs, the QTL for five main stem segments could be detected by both algorithms, which explained that the range of phenotypic variation was 8.6% -27.0%. One stem crude QTL explained that the range of phenotypic variation was 9.0% -11.0 %; Six stem heavy QTL, explaining the range of phenotypic variation of 6.0% -39.0%. Three QTLs for main stem segments could be detected in more than two years, which explained that the range of phenotypic variation was 8.0% -60.2%; two stem QTLs explained that the range of phenotypic variation was 10.0% -23.0%; for more than two years Rough stem QTLs were not detected repeatedly. 【Conclusion】 By comparing the QTLs for the number of main stems, stems and stems, we found that there was a large genetic correlation between these traits.