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目的 研究转染人体肿瘤坏死因子 α(huTNF α)、白介素 2 (hIL 2 )基因对肺癌细胞耐药基因MDR1、LRP表达的影响 ,探讨基因治疗逆转肺癌多药耐药的可行性。方法 通过阳离子脂质体将克隆的huTNF α和hIL 2基因导入肺癌细胞系A5 49、GLC 82、H44 6、H460 ,经筛选阳性单克隆 ,用半定量RT PCR方法检测转染目的基因前后肺癌细胞系MDR1、LRP基因在mRNA水平的表达情况。结果 MDR1在A5 49、GLC 82、H44 6、H460 ,LRP在A5 49、GLC 82、H 460中均呈阳性表达 ;转染huTNF α目的基因后各肺癌细胞系MDR1、LRP基因的表达无影响 ,而转染hIL 2目的基因能够明显抑制A5 49、H44 6、H 460细胞系MDR1的表达。结论 MDR1、LRP基因在肺癌细胞系中的阳性表达与肺癌的固有耐药性有关 ;huTNF α、hIL 2目的基因能够在被转染细胞中获得表达 ,而且转染hIL 2目的基因能够明显抑制MDR1在A5 49、H44 6、H460的表达。该结果不仅为探讨肺癌多药耐药性的病理机制提供了一个新的线索 ,而且为基因治疗逆转肺癌的多药耐药性提供了一个新的实验依据。
Objective To investigate the effects of human tumor necrosis factor α (huTNF α) and interleukin 2 (hIL 2) gene on the expression of multidrug resistance genes (MDR1 and LRP) in lung cancer cells and to explore the feasibility of gene therapy in reversing multidrug resistance of lung cancer. Methods Human lung cancer cell lines A549, GLC82, H446 and H460 were transfected with the gene of human liposome through the method of semi-quantitative RT-PCR. Department of MDR1, LRP gene expression at the mRNA level. Results The expression of MDR1 in A549, GLC 82, H44 6, H460 and LRP was positive in A549, GLC 82 and H 460, respectively. The expression of MDR1 and LRP gene in lung cancer cell lines transfected with huTNF α gene had no effect, Transfection of hIL 2 gene could significantly inhibit MDR1 expression in A549, H446 and H460 cell lines. Conclusions The positive expression of MDR1 and LRP genes in lung cancer cell lines is related to the inherent drug resistance of lung cancer. The target genes of huTNF α and hIL 2 can be expressed in transfected cells, and the transfected hIL 2 gene can significantly inhibit MDR1 Expression of A549, H446, H460. This result not only provides a new clue to explore the pathological mechanism of multidrug resistance in lung cancer, but also provides a new experimental basis for gene therapy to reverse the multidrug resistance of lung cancer.