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我们分离和提纯了人绒毛膜mRNA和兔脑mRNA,为构建人绒毛膜cDNA文库和兔脑cDNA文库配备了必要的条件。一、材料和方法 1.材料: (1)同位素:~(35)S-甲硫氨酸(0.74mCi/ml,中国科学院生物物理研究所);~3H-甲硫氨酸(mCi/ml,Sigma公司);Oligo(dT)-纤维素
We isolated and purified human chorionic mRNA and rabbit brain mRNA and provided the necessary conditions for constructing human chorionic cDNA library and rabbit brain cDNA library. Materials and methods 1. Materials: (1) isotopes: ~ (35) S-methionine (0.74 mCi / ml, Institute of Biophysics, Chinese Academy of Sciences); ~ 3H- methionine (mCi / Sigma); Oligo (dT) - cellulose