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目的:观察实验豚鼠胆囊组织调宁蛋白(Cap)表达水平的变化,探讨养肝柔肝中药防治胆石病的作用机制。方法:将受试豚鼠随机分为4组,即正常组、模型组、养肝柔肝中药组(中药组)、熊去氧胆酸西药组(西药组)。后3组采用高胆固醇致石食饵诱发法建立豚鼠胆结石动物模型,其中中药组、西药组在造模的同时分别给予相应药物干预;连续7周后,分别对实验豚鼠胆结石生成情况,胆囊组织Cap蛋白的表达进行观察。结果:养肝柔肝中药组能有效防止胆结石的生成,与模型组比较有显著性差异(P<0.01),并且其效果优于熊去氧胆酸西药组(P<0.05)。模型组动物胆囊组织Cap蛋白表达水平较正常组显著升高(P<0.01);养肝柔肝中药组和熊去氧胆酸西药组胆囊组织Cap蛋白表达水平虽均高于正常组(分别为P<0.05,P<0.01),但较模型组明显降低(均P<0.01),且养肝柔肝中药组Cap蛋白表达水平较低,但与熊去氧胆酸西药组无显著性差异(P>0.05)。结论:养肝柔肝中药能显著降低胆结石的形成,其作用机制可能与下调胆囊平滑肌组织Cap蛋白的水平,提高胆囊平滑肌收缩能力有关。
OBJECTIVE: To observe the changes of the expression level of calponin (Cap) in the gallbladder tissue of experimental guinea pigs and to explore the mechanism of action of Yanggan Rougan traditional Chinese medicine in the prevention and treatment of cholelithiasis. Methods: The guinea pigs were randomly divided into 4 groups: normal group, model group, Yanggan Rougan Chinese medicine group (Chinese medicine group), and ursodeoxycholic acid western medicine group (Western medicine group). In the last 3 groups, animal models of gallstones in guinea pigs were induced by high cholesterol-induced food predation. The traditional Chinese medicine group and western medicine group were given the corresponding drug interventions at the same time when the model was established. After 7 weeks of continuous operation, gallstone formation in the guinea pigs was determined. Tissue Cap protein expression was observed. Results: The “Yanggan Rougan” traditional Chinese medicine group can effectively prevent gallstone formation, which is significantly different from that of the model group (P <0.01), and its effect is superior to that of the ursodeoxycholic acid western medicine group (P <0.05). The expression of Cap protein in the gallbladder of the animal model group was significantly higher than that of the normal group (P<0.01). The expression of Cap protein in the gallbladder of the Nourishing Liver Rougan Chinese medicine group and the Xiong Deoxycholic acid Western medicine group was higher than the normal group (respectively P<0.05, P<0.01), but it was significantly lower than that in the model group (all P<0.01), and Cap protein expression level was lower in the Yanggan Rougan Chinese medicine group, but there was no significant difference with the western medicine group of ursodeoxycholic acid ( P>0.05). Conclusion: The traditional Chinese medicine with nourishing liver and softening liver can significantly reduce the formation of gallstones. The mechanism may be related to the down-regulation of Cap protein level in gallbladder smooth muscle and the enhancement of gallbladder smooth muscle contraction.