γδＴ淋巴细胞在人外周血单个核细胞中的含量不超过１０ ％ ， 而研究γδＴ淋巴细胞的功能需要大量的γδＴ淋巴细胞。为此， 采用磁珠（ｍａｇｎｅｔｉｃ ｍｉｃｒｏｂｅａｄｓ）阳性选择（ｐｏｓｉｔｉｖｅｓｅｌｅｃｔｉｏｎ） 法分离和纯化γδＴ淋巴细胞， 纯化可达９９％ 左右； 同时对富集的γδＴ淋巴细胞在体外的增殖和细胞毒效应进行观察， 此法快速、有效、纯度高， 对γδＴ淋巴细胞功能影响较小。为γδＴ淋巴细胞的研究和应用提供了便利。 γδT lymphocytes in human peripheral blood mononuclear cells in the content of not more than 10%, while the study of γδ T lymphocyte function requires a large number of γδ T lymphocytes. For this purpose, the positive selection of magnetic microbeads was used to separate and purify γδT lymphocytes, and the purification was up to 99%. Meanwhile, the proliferation and cytotoxicity of enriched γδT lymphocytes in vitro were observed. Fast, effective, high purity, less effect on γδ T lymphocyte function. It provides convenience for the research and application of γδT lymphocytes.