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目的 探讨上海地区近 5年来急性呼吸道感染患儿病毒病原和支原体病原感染状况。方法 采用系列呼吸道病毒单克隆抗体桥联酶标法或聚合酶链反应技术 ,对上海地区 5年中的急性呼吸道感染患儿 5 496例标本进行病毒学或肺炎支原体 -DNA检测。结果 检出病毒阳性 2 2 5 7例 ,阳性率 41 0 7%以上 ,肺炎支原体 -DNA检出率 16 2 % (5 72 / 35 38)。结论 连续 5年中上海地区有 2次急性呼吸道病毒感染发病高峰 (1994年和 1998年 ) ,病毒性感染流行季节主要在每年 1～ 2月和 11～ 12月
Objective To investigate the prevalence of viral pathogens and mycoplasmas in children with acute respiratory infection in Shanghai in the recent 5 years. Methods A total of 5 496 children with acute respiratory infection in Shanghai were enrolled in the study of virology or Mycoplasma pneumoniae (Mycoplasma pneumoniae) using a series of monoclonal antibodies against respiratory syncytial virus or polymerase chain reaction (PCR). Results A total of 22 57 positive cases were detected, with a positive rate of 41 0 7%. The detection rate of Mycoplasma pneumoniae-DNA was 162% (5 72/35 38). Conclusions The incidence of acute respiratory virus infection in Shanghai in two consecutive years (1994 and 1998) has been the highest in five consecutive years. The prevalence of viral infection is mainly in January ~ February and November ~ December