OBJECTIVE:To investigate the anti-bacterial and anti-viral effects of Fengreqing oral liquid(风热清口服液,FRQ) in vitro and in vivo.METHODS:The minimum inhibitory concentra-tions of Fengreqing Oral Liquid against six gram-positive bacteria (Staphylococcus aureus,Streptococcus mutans,Peptostreptococcus anaero-bius,Hemolytic streptococcus,Streptococcus pneu-moniae,Klebsiella pneumoniae),seven gram-nega-tive bacteria (Escherichia coli,Actinobacillus actino-mycetemcomitans,Porphyromonas gingivalis,Hae-mophilus influenzae,Helicobacter pylori,Pseudo-monas aeruginosa,Gardnerella vaginalis) and Can-dida albicans were detected by the paper disc diffu-sion method.The inhibition rate of A/PuertoRico/8/34(H1N1) (PR8) influenza virus in different concentra-tions of Fengreqing oral solution was detected by chicken embryo method.CCK8 method was used to detect the half-cell infection of RSV,VSV and CVB3.The effect of FRQ on the survival curve of mice was detected by using co-infection model of Streptococcus pneumoniae and influenza virus.RESULTS:In vitro,FRQ can inhibit Actinobacillus ac-tinomycetemcomitans,Helicobacter pylori,Gard-nerella vaginalis,Staphylococcus aureus,Strepto-coccus mutans and Streptococcus pneumoniae and has an antiviral effect on the envelope virus H1N1.In vivo,Fengreqing oral solution had thera-peutic effect on influenza-Streptococcus pneu-moniae co-infection in mice,significantly improv-ing the survival rate of mice.The medium dose and low dose FRQ significantly prolonged the survival time of mice.CONCLUSION:FRQ has good anti-bacterial and an-ti-viral effects in vivo and in vitro.