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目的:体外培养大鼠骨髓间充质干细胞(MSCs),探讨其对移植肾诱导免疫耐受作用。方法:无菌条件下取大鼠股骨、胫骨,用直接贴壁法分离纯化MSCs,体外扩增,在注射MSCs的基础上,行同种异体肾移植,实验大鼠分为注射大鼠骨髓MSCs肾移植组(I组)和单纯肾移植组(Ⅱ组),均未用任何免疫抑制剂,术后3d结扎受体右肾动脉(左侧为移植肾);观察右肾动脉结扎术后受体存活时间和右肾术后移植肾的病理变化。结果:I组大鼠生存期(15.50±6.35)d较Ⅱ组(6.50±3.ll)d明显延长,且前者移植肾形态学观察和功能状态均明显优于后者。结论:MSCs在体外很容易分离培养和扩增,MSCs对早期移植肾具有保护作用。
OBJECTIVE: To culture rat bone marrow mesenchymal stem cells (MSCs) in vitro and investigate the effect of inducing immune tolerance on transplanted kidneys. METHODS: Rat femur and tibia were obtained under aseptic conditions. MSCs were separated and purified by direct adherent method and expanded in vitro. Allogeneic renal transplantation was performed on the basis of MSCs injection. The rats were divided into 3 groups: In the kidney transplantation group (group I) and the simple kidney transplantation group (group II), no immunosuppressive agents were used. The recipient right renal artery (left kidney graft) was ligated after 3d and the right renal artery was ligated Survival time and pathological changes of renal grafts after right kidney surgery. Results: The survival time of group I was significantly longer than that of group II (6.50 ± 3.11) d (15.50 ± 6.35) d, and the morphological observation and functional status of the former were superior to the latter. CONCLUSION: MSCs can be easily isolated and cultured in vitro and MSCs have a protective effect on early graft kidney.