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蛋白甘油是切片制作过程中常用的一种附贴剂,它可使切片紧贴于载玻片上。但多年的工作实践中,发现虽然采用蛋白甘油做为附贴剂,但仍有脱片现象发生,同时蛋白甘油使切片背景红染,影响了切片的清晰度。为了改进上述现象,简化制片手续,提高工作效率,考虑是否可省去蛋白甘油这一程序(可省去调制蛋白甘油,片前将蛋白甘油涂抹在玻片上,染色后封片前擦去多余的红染蛋白甘油等手续。)。载玻片如果非常光洁,即使没有蛋白
Glycerin, a common adjunct to slicing, makes it possible to attach the slice to a glass slide. However, many years of work practice found that although the use of protein glycerin as an adjunct, but there is still the phenomenon of delamination, while the protein glycerol slice background red dye, affecting the clarity of the slice. In order to improve the above phenomenon, simplify the preparation procedures and improve work efficiency, consider whether the process of protein glycerin can be omitted (which can omit the modulation of glycerol, glycerol on the slide before the film smear, Red stained protein glycerol and other procedures.). Slides, if very clean, even without protein