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以LDH酶释放法,K562细胞及胃癌细胞(SGC-7901)为靶细胞分别测定20例健康高龄者(平均年龄为76.8±6.01岁)的外周血淋巴细胞中的NK细胞及胃癌杀伤细胞活力;并以L929为靶细胞,MTT细胞毒活力法测定经IL—2,LPS分别诱导活化PBL合成分泌TNF活力,并与健康中青年(平均年龄为35.1±6.06岁)相比较,以探求老年人的细胞免疫及对肿瘤的防御功能。结果发现离龄组的杀伤细胞活力为33.04±6.36,低于健康中青年组(38.12±5.67;P<0.05),但经IL-2或LPS活化诱导分泌的TNF活力分别为52.45±4.72%、53.78±8.86%,均明显高于中青年组(分别为44.47±5.69%及47.87±5.50%),差异均显著。提示高令组健康人群的肿瘤杀伤细胞活力均降低,但TNF分泌活力增高,可能补偿了部分免疫缺陷。
K562 cells and gastric cancer cells (SGC-7901) were used as target cells to measure NK cell activity and gastric cancer cell killing activity in 20 healthy elderly (mean age: 76.8 ± 6.01) peripheral blood lymphocytes by LDH enzyme release assay. L929 was used as target cell. The activity of TNF induced by activated PBL induced by IL-2 and LPS was measured by MTT cytotoxicity assay, and compared with healthy middle-aged (mean age 35.1 ± 6.06 years old) Cellular immunity and defense against tumors. The results showed that the lethal group had an activity of 33.04 ± 6.36, which was lower than that of the healthy group (38.12 ± 5.67; P <0.05), but the activity of TNF induced by IL-2 or LPS was 52.45 ± 4.72% 53.78 ± 8.86%, which were significantly higher than those in middle-aged group (44.47 ± 5.69% and 47.87 ± 5.50%, respectively). It suggested that the tumor-killing cell vitality of Gaoling healthy population was decreased, but the TNF secretion activity was increased, which may compensate for some immunodeficiency.