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目的:观察去势大鼠卵巢移植后海马神经元数量变化及雌激素受体β(ER-β)和神经颗粒素(NG)表达的变化,评价卵巢移植对中枢神经的保护作用。方法:成年雌性SD大鼠行双侧卵巢切除术(OVX)后,在肾被膜下移植出生3日内乳鼠的卵巢,于干预后的4、7、14、21 d和28 d,通过Nissl染色计数海马神经元的数量,采用免疫组化法观察海马神经元ER-β和NG蛋白的表达情况,并与OVX组和正常对照组比较。结果:与OVX组相比,卵巢移植组随移植时间的延长,海马神经元的数量逐步恢复;ER-β和NG蛋白的表达在海马各区均有所升高(P<0.05)。结论:卵巢移植可以防止海马神经元数量的减少,并促进ER-β和NG表达,发挥雌激素对中枢神经系统的保护作用。
OBJECTIVE: To observe the change of hippocampal neurons and the expression of estrogen receptor β (ER-β) and neurogranin (NG) after ovariectomy in ovariectomized rats to evaluate the protective effect of ovarian transplantation on the central nervous system. Methods: Adult female SD rats were ovariectomized (OVX) and then transplanted into the ovary of neonatal rats within 3 days after their birth. The ovaries were stained by Nissl at 4, 7, 14, 21 d and 28 d after the intervention The number of hippocampal neurons was counted. The expression of ER-βand NG protein in hippocampal neurons was observed by immunohistochemistry and compared with OVX group and normal control group. Results: Compared with OVX group, the number of hippocampal neurons recovered gradually with the prolongation of transplantation time in ovarian transplantation group. The expression of ER-β and NG protein in hippocampus increased (P <0.05). Conclusion: Ovarian transplantation can prevent the decrease of the number of hippocampal neurons, promote the expression of ER-β and NG, and exert the protective effect of estrogen on the central nervous system.