论文部分内容阅读
大鼠肝大部切除后和D-氨基半乳糖肝中毒后第1~7天,逐日取肝组织及血液样本,观察两种类型损伤后肝再生中肝细胞的增殖分化,以及血清甲胎蛋白(AFP)与白蛋白(ALB)浓度变化及其免疫细胞化学变化。结果表明:1.肝大部切除后肝细胞分裂高峰(1.5%)出现于术后第2天,半乳糖肝中毒组的高峰在第3天,且峰值仅为前者的一半(0.8%)。2.半乳糖肝中毒后,门管区和小叶周边带出现许多小型细胞,而大部切除后的肝内无此种小型细胞。3.AFP与ALB免疫细胞化学观察表明,两种损伤后肝再生中均有AFP阳性肝细胞,于第2~3天增多,第4天后渐少。部分小型细胞呈AFP阳性。电镜下见粗面内质网、滑面内质网、高尔基复合体及核周隙等处显示AFP阳性。ALB阳性细胞第1~3天较少,于第4天起渐增多。4.血清AFP含量于第1天开始升高,第4天达高峰,此后下降,ALB含量变化恰与AFP相反,第3天下降至最低点,此后回升。
After partial hepatectomy and D-galactosamine hepatotoxicity 1 to 7 days, liver tissues and blood samples were taken daily to observe the proliferation and differentiation of hepatocytes in liver regeneration after two types of injury, and the serum levels of alpha-fetoprotein (AFP) and albumin (ALB) concentrations and immunocytochemical changes. The results showed that: (1) The peak of hepatocyte division (1.5%) occurred on the second day after subtotal hepatectomy. The peak of galactose hepatotoxicity group was on the third day and the peak value was only half (0.8%) of the former. 2. Galactosyne liver poisoning, portal area and lobular zone appeared many small cells, and most of the resected liver without such small cells. Immunocytochemistry of AFP and ALB showed that AFP-positive hepatocytes were present in both liver regeneration after injury, increasing on day 2 to 3 and decreasing on day 4. Some small cells were AFP-positive. Electron microscope, see the rough endoplasmic reticulum, endoplasmic reticulum, Golgi complex and the perivascular space and other places showed AFP-positive. The number of ALB positive cells decreased from the first day to the third day and gradually increased on the fourth day. Serum AFP levels began to rise on the first day, peaked on the fourth day, and then decreased. The ALB content changed just opposite to AFP, and dropped to the lowest point on the third day and then rose again.