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目的 :探讨蓝氏贾第鞭毛虫大、小型包囊存在的原因。方法 :用分子生物学技术对 3株贾第虫 (CH2、CH3、CH4)进行 tim基因扩增和序列测定。结果 :经与 Gen Bank登记虫株比较和用 DNAstar软件处理的结果表明 ,此 3株贾第虫的基因型属 2型 (JH型 )。结论 :大、小型包囊在形态学上经过统计学处理显示有显著差异 ,但与基因型无关。
Objective: To investigate the causes of large and small cysts in Giardia lamblia. Methods: Three strains of Giardia (CH2, CH3, CH4) were amplified by tim gene and sequenced using molecular biology techniques. Results: The genotypes of Giardia were genotype 2 (JH type) compared with those registered with Gen Bank and DNAstar software. CONCLUSIONS: Large and small cysts showed statistically significant differences in morphology but not genotypes.