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建立了早胜牛心肌中牛磺酸的高效液相检测方法并优化其提取工艺。以异硫氰酸苯酯为衍生剂,HPLC法检测牛磺酸浓度。采用Shim-pack VP-ODS C18色谱柱,流动相流速1.0 m L/min,检测波长254 nm,柱温36℃。以早胜牛心肌为原料,研究料液比、提取时间、水解时间、提取温度对牛磺酸提取率的影响,通过响应面分析法优化其最佳提取工艺。牛磺酸线性范围为0.37~90μg/m L,相对标准偏差(RSD)在1.5%~2.3%(n=4)之间。当料液比1∶5、提取时间55 min、水解时间20.5 h、提取温度85℃时,牛磺酸最高提取率可达3.76 mg/g。方法对于早胜牛中牛磺酸提取和检测应用具有一定参考价值。
The method of high performance liquid phase detection of taurine in Zaisheng bovine myocardium was established and its extraction process was optimized. Using phenylisothiocyanate as a derivatization reagent, the taurine concentration was determined by HPLC. A Shim-pack VP-ODS C18 column was used with a mobile phase flow rate of 1.0 mL / min. The detection wavelength was 254 nm and the column temperature was 36 ℃. The effect of feed ratio, extraction time, hydrolysis time and extraction temperature on the extraction rate of taurine was studied. The optimal extraction process was optimized by response surface methodology. The linear range of taurine was 0.37 ~ 90μg / m L with relative standard deviation (RSD) of 1.5% -2.3% (n = 4). When the ratio of solid to liquid was 1: 5, the extraction time was 55 min, the hydrolysis time was 20.5 h and the extraction temperature was 85 ℃, the highest extraction rate of taurine was 3.76 mg / g. The method has certain reference value for taurine extraction and detection application in Zaishenghu cattle.