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目的观察serglycin稳定干扰后对鼻咽癌高转移细胞顺铂敏感性的影响,并初步探究其机制。方法建立serglycin稳定干扰的鼻咽癌高转移细胞株,qRT-PCR和Western blot检测serglycin的表达情况。四甲基偶氮唑蓝法测定顺铂对稳定株细胞的IC50;增殖曲线实验观察在顺铂压力下细胞增殖的差异;克隆形成实验观察细胞克隆形成能力的差异;Annexin V/PI双染检测细胞凋亡。qRT-PCR检测稳定株细胞中干性基因的表达情况。结果 Serglycin稳定干扰的鼻咽癌高转移细胞株成功建立,细胞形态发生改变。四甲基偶氮唑蓝法测得serglycin稳定干扰细胞的顺铂IC50显著低于对照组细胞,增殖抑制率高于对照组,药物作用下克隆形成能力减弱,流式凋亡比例增高。Serglycin稳定干扰后干性基因表达下调。结论 Serglycin稳定干扰可以增加鼻咽癌高转移细胞对顺铂的敏感性。
Objective To investigate the effect of serglycin on the sensitivity of cisplatin-induced nasopharyngeal carcinoma cells to metastasis, and to explore its mechanism. Methods Serglycin stable interfering nasopharyngeal carcinoma cell line was established. The expression of serglycin was detected by qRT-PCR and Western blot. IC50 of cisplatin on stable cells was determined by MTT method. Differences of cell proliferation under cisplatin pressure were observed by proliferation curve assay. Differences of cell clonality were observed by clonogenic assay. Annexin V / PI double staining Apoptosis. qRT-PCR was used to detect the expression of dry genes in stable cells. Results Serglycin stable interference of nasopharyngeal carcinoma cell lines were successfully established, the cell morphology changed. The IC50 of cisplatin of serglycin stable interfering cells measured by MTT assay was significantly lower than that of control cells, the inhibition rate of proliferation was higher than that of control group, and the clonogenic capacity decreased with the increase of flow cytometry. Down regulation of dry gene expression after stable interference of Serglycin. Conclusion Stable interference of Serglycin can increase the sensitivity of NPC cells to Cisplatin.