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目的:探讨导流杂交基因芯片技术在宫颈癌人乳头状瘤病毒(HPV)分型检测中的临床应用。方法:采用导流杂交基因芯片技术对1 169例宫颈癌脱落细胞标本进行HPV检测并分型。结果:1 169例宫颈癌中HPV感染率为91.70%(1 072/1 169),其中单一感染占77.16%(902/1 169),多重感染占14.54%(170/1 169),检出率较高的HPV基因型有HPV16(62.10%)、HPV18(10.95%)、HPV58(10.52%)、HPV52(7.96%)和HPV33(3.93%)。结论:导流杂交基因芯片技术可检测HPV多种基因型,对HPV感染基因型的鉴别及宫颈癌的预防和治疗具有较好的临床应用价值。中华肿瘤防治杂志,2010,17(11):812-814
Objective: To investigate the clinical application of flow-through hybridization gene microarray in the detection of human papillomavirus (HPV) in cervical cancer. Methods: The flow cytometry (IHC) microarray technique was used to detect and classify HPV in 1 169 cases of cervical cancer exfoliated cells. Results: The prevalence of HPV infection in 1 169 cases of cervical cancer was 91.70% (1072/1 169), of which 77.16% (902/1 169) were single infection and 14.54% (170/1 169) with multiple infection, the detection rate The higher HPV genotypes were HPV16 (62.10%), HPV18 (10.95%), HPV58 (10.52%), HPV52 (7.96%) and HPV33 (3.93%). CONCLUSION: Diversified genotypes of HPV can be detected by flow-through hybridization gene chip technology, and it has a good clinical value for identification of HPV genotypes and prevention and treatment of cervical cancer. Chinese Journal of Cancer Prevention and Treatment, 2010,17 (11): 812-814