论文部分内容阅读
以一年生艾纳香种子苗为实验材料,用一水合氯化钙提供钙元素,在冬季艾纳香生长迟缓期进行3次施肥,测定艾纳香的株高、地径、叶长和叶宽等生长指标以及生物量。采用紫外可见分光光度法测定艾纳香不同部位中总黄酮的相对含量,并计算总黄酮的绝对含量;采用GC测定艾纳香叶片中l-龙脑的相对含量,并计算l-龙脑的绝对含量。结果表明:钙元素极显著增加了冬季生长迟缓期的艾纳香叶、茎和根生物量,其中5 g/L钙处理组的艾纳香叶生物量极显著高于其他3个处理组,10、15 g/L钙处理下的叶生物量极显著高于空白对照组(CK),分别是对照的3.03倍和2.65倍。钙元素的施加抑制了艾纳香不同部位总黄酮相对含量的积累,然而显著增加了总黄酮绝对含量。5 g/L钙处理组的l-龙脑相对含量和绝对含量最高,分别为0.22%和0.22 g,与10、15g/L钙和CK组相比,分别增加了37.50%、22.22%、37.50%和100%、100%、450%。在冬季艾纳香生长迟缓期施加钙元素可以显著促进艾纳香叶、茎和根生物量的积累,提高总黄酮和l-龙脑的绝对含量。
The annual seedlings of Artemisia selengensis were used as experimental material, calcium was provided by calcium chloride monohydrate, and fertilization was performed three times during the retarded growth period of Anemones in winter. The plant height, diameter, leaf length and leaf width And other growth indicators and biomass. The relative content of total flavonoids in different parts of Anemia fragrans was determined by UV-visible spectrophotometry, and the absolute content of total flavonoids was calculated. The relative content of l-borneol in leaves of Anemarcus fragrans was determined by GC, Absolute content. The results showed that calcium significantly increased the leaf, stem and root biomass of A. furnacalis in the retarding period of winter growth. The biomass of A. napus in 5 g / L calcium treatment group was significantly higher than that in the other three treatments. The leaf biomass under 10 and 15 g / L calcium treatment was significantly higher than that of CK (3.03 and 2.65 folds, respectively). The application of calcium inhibited the accumulation of total flavonoids in different parts of Anemia, but significantly increased the absolute content of total flavonoids. The relative content and absolute content of l-borneol in 5 g / L calcium treatment group were the highest, 0.22% and 0.22 g respectively, which increased by 37.50%, 22.22% and 37.50% respectively compared with 10,15 g / L calcium and CK % And 100%, 100%, 450%. The application of calcium in the retarded growth phase of Anemone in winter can significantly promote the accumulation of biomass of leaves, stems and roots, and increase the absolute content of total flavonoids and l-borneol.