目的克隆维甲酸诱导的人肺腺癌ＧＬＣ８２细胞分化相关基因。方法在全反式维甲酸诱导人肺腺癌ＧＬＣ８２细胞系分化的基础上，采用ｍＲＮＡ差异显示技术，对这个细胞系以全反式维甲酸诱导前及诱导后８小时、２４小时和４天的细胞基因表达差异情况进行分析。结果在全反式维甲酸诱导前后的细胞之间存在明显的基因表达差异。有些基因经维甲酸（ＲＡ）诱导后持续表达或瞬时表达，而有些基因经ＲＡ作用后表达水平降低或完全被抑制。经克隆筛选，获得了一些经全反式维甲酸诱导激活或抑制的差异表达基因片段，对其中的３个片段进行了序列分析和同源性比较。结论全反式维甲酸具有调控分化相关基因表达与否的重要作用，由ＲＡ诱导的肿瘤细胞再分化是一个多基因参与的过程 Objective To clone retinoic acid-induced human lung adenocarcinoma GLC82 cell differentiation-related genes. Methods Based on the differential expression of all trans retinoic acid-induced human lung adenocarcinoma GLC-82 cell line, mRNA differential display technology was used. This cell line was treated with all-trans retinoic acid before and 8 hours, 24 hours and 4 hours after induction. Days of cell gene expression differences were analyzed. Results There were significant gene expression differences between cells before and after induction of all-trans retinoic acid. Some genes are continuously or transiently expressed after induced by retinoic acid (RA), and some genes are reduced or completely inhibited by RA. After cloning and screening, some differentially expressed gene fragments were induced or inhibited by all-trans retinoic acid. Sequence analysis and homology comparison were performed on the three fragments. Conclusion All-trans retinoic acid has an important role in regulating the expression of differentiation-related genes. The re-differentiation of tumor cells induced by RA is a multi-gene participation process.