论文部分内容阅读
目的:比较中药天龙粉发酵前后水溶性活性物质的含量。方法:采用超声水提取法分别提取天龙粉发酵前后所含的水溶性活性成分,通过改良Lowry法测定其蛋白质含量、苯酚-硫酸法测定其多糖含量、定磷法测定其核酸含量。结果:天龙粉发酵前蛋白含量为68.5730mg/g,发酵后为130.6559mg/g;天龙粉发酵前多糖含量为6.5771mg/g,发酵后为13.4492mg/g;而天龙粉发酵前核酸含量为17.7146mg/g,发酵后为9.1373mg/g。结论:天龙粉发酵前的核酸含量较高,而发酵后的蛋白质和多糖含量较高。
Objective: To compare the content of water-soluble active substance before and after fermentation of Chinese medicine Tianlong powder. Methods: The water-soluble active ingredients before and after fermentation were extracted by ultrasonic water extraction. The protein content was determined by modified Lowry method. The content of polysaccharide was determined by phenol-sulfuric acid method. The results showed that the content of protein before fermentation was 68.5730mg / g and 130.6559mg / g after fermentation. The content of polysaccharide before fermentation was 6.5771mg / g and 13.4492mg / g after fermentation. The content of nucleic acid before fermentation was 17.7146 mg / g and 9.1373 mg / g after fermentation. Conclusion: The content of nucleic acid of Tianlong meal before fermentation is high, while the content of protein and polysaccharide after fermentation is high.