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为探讨抗ＡＰＯ－１单抗处理的金黄色葡萄球菌肠毒素Ｂ（ＳＥＢ）活化的淋巴细胞胞浆中游离Ｃａ２＋浓度的变化，用形态学观察、流式细胞光度术观测了鼠抗人ＡＰＯ－１单克隆抗体对ＳＥＢ活化不同天数的人外周血淋巴细胞凋亡的诱导作用，同时采用Ｆｕｒａ－２／ＡＭ荧光探针检测了ＡＰＯ－１单抗处理的淋巴细胞胞浆内游离Ｃａ２＋浓度。结果表明，抗ＡＰＯ－１单抗对ＳＥＢ活化１ｄ和对照组淋巴细胞无明显的促凋亡作用，但对ＳＥＢ活化５ｄ的淋巴细胞不仅可明显促进其凋亡，而且可使其胞浆游离Ｃａ２＋浓度升高，而Ｚｎ２＋则抑制了抗ＡＰＯ－１单抗的凋亡诱导作用。由此可见，抗ＡＰＯ－１单抗与其特异性抗体结合后可能是通过使胞浆中游离Ｃａ２＋浓度升高，激活了Ｃａ２＋／Ｍｇ２＋依赖的内源性核酸内切酶而导致ＳＥＢ活化的淋巴细胞凋亡。
In order to investigate the changes of free Ca2 + concentration in cytoplasm of lymphocytes activated by APO-1 monoclonal antibody against Staphylococcus aureus enterotoxin B (SEB), morphological observation and flow cytometry were used to observe the effect of mouse anti-human APO- 1 monoclonal antibody on the apoptosis of human peripheral blood lymphocytes activated by SEB for different days, and the concentration of free Ca2 + in cytoplasm of APO-1-treated lymphocytes was detected by Fura-2 / AM fluorescent probe. The results showed that anti-APO-1 mAb had no obvious effect on promoting apoptosis of lymphocytes activated by SEB on the 1st day, but not on the 5th day after activation of SEB. Not only the apoptosis of SEB-treated lymphocytes but also the cytoplasmic free Ca2 + Concentration, while Zn2 + inhibited the induction of APO-1 mAb apoptosis. Thus, the combination of anti-APO-1 mAb and its specific antibody may be through activation of Ca2 + / Mg2 + -dependent endogenous endonuclease and activation of SEB-activated lymphocytes by increasing the concentration of free Ca2 + in the cytoplasm Apoptosis.