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选择２４ 例未经治疗的喉鳞状细胞癌患者术后新鲜肿瘤标本，通过流式细胞仪（ＦＣＭ）测定ＤＮＡ 含量的３ 个主要参数，异倍体（ａｎｅｕｐｌｏｉｄ）、ＤＮＡ 指数（ＤＮＡ Ｉｎｄｅｘ，ＤＩ）和Ｓ期细胞比例（Ｓ－ｐｈａｓｅ ｆｒａｃ－ｔｉｏｎ，ＳＰＦ），探索３ 个参数与肿瘤组织学分级、临床分期以及淋巴结转移之间的关系。结果表明，２４ 例喉鳞状细胞癌中，异倍体检出率为８３．３％ （２０／２４）。ＤＩ与肿瘤组织学分级和淋巴结转移无关，与肿瘤临床分期有关（Ｐ＜ ０．０５）。ＳＰＦ与肿瘤组织学分级无关，与临床分期及淋巴结转移有关（Ｐ＜ ０．０１，Ｐ＜ ０．０５）。提示：（１）ＤＮＡ异倍体是喉鳞状细胞癌的重要特征之一；（２）ＤＩ和ＳＰＦ可作为预测喉鳞状细胞癌生物学特性的重要指标
Twenty-four patients with untreated laryngeal squamous cell carcinoma were enrolled in this study. Fresh tumor specimens were collected from the patients with laryngeal squamous cell carcinoma. The three main parameters of DNA content were determined by flow cytometry (FCM), aneuploid, DNA Index (DI) ) And S-phase frac-tion (SPF). The relationship between the three parameters and tumor histological grade, clinical stage and lymph node metastasis was explored. The results showed that in 24 cases of laryngeal squamous cell carcinoma, the detection rate of aneuploidy was 83.3% (20/24). DI was not related to tumor histological grade and lymph node metastasis, but also to clinical staging (P <0.05). SPF had no correlation with tumor histological grade, and was related to clinical stage and lymph node metastasis (P <0.01, P <0.05). Tip: (1) DNA aneuploidy is one of the important features of laryngeal squamous cell carcinoma; (2) DI and SPF can be used as an important index to predict the biological characteristics of laryngeal squamous cell carcinoma