Environmental stress factors could lead to the excess generation of reactive oxygen species(ROS) that induces various forms of skin damage related to oxidative stress. Polyhydroxylated fullerene derivative C_(60)(OH)_n, acting as an effective agent for prevention of skin aging, is widely used in the lotion and sunscreens in the field of cosmetics, but rarely used in the masks. In this study, we prepared C_(60)(OH)_n loaded nanofibrous membranes to protect human keratinocyte cells from ROS-associated damage and suppress the elevation of intracellular ROS and Ca~(2+) along with the apoptotic cell death. Two FDAapproved biodegradable polymers, PLGA and PCL, have been used for making the electrospun nanofibers,with C_(60)(OH)_n added to the polymers as an antioxidant. The nanofibrous membranes with good biocompatibility might be potentially applied in clinical practice to reduce skin aging. Environmental stress factors could lead to the excess generation of reactive oxygen species (ROS) that induces various forms of skin damage related to oxidative stress. Polyhydroxylated fullerene derivative C_ (60) (OH) _n, acting as an effective agent for prevention of skin aging , is widely used in the lotion and sunscreens in the field of cosmetics, but rarely used in the masks. In this study, we prepared C_ (60) (OH) _n loaded nanofibrous membranes to protect human keratinocyte cells from ROS-associated damage and suppress the elevation of intracellular ROS and Ca ~ (2+) along with the apoptotic cell death. Two FDA applied biodegradable polymers, PLGA and PCL, have been used for making the electrospun nanofibers, with C 60 (OH) _n added to the polymers as an antioxidant. The nanofibrous membranes with good biocompatibility might be potentially applied in clinical practice to reduce skin aging.