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目的 观察HCVC区基因在SP2 / 0细胞及荷瘤小鼠瘤内的表达情况 .方法 构建pEF HCVC重组质粒 ,转染SP2 / 0细胞 ,经G4 18进行筛选 ,建立稳定转染pEF HCVC的SP2 / 0细胞系 .并将转染后的SP2 / 0细胞接种于BALB/c小鼠皮下产生浆细胞瘤 .结果 用斑点杂交方法检测转染后的细胞 ,有HCVmRNA .免疫组化检测瞬时转染以及稳定转染的SP2 / 0细胞涂片均发现存在有阳性信号 .另外 ,对瘤组织进行免疫组化检测 ,发现有HCVC蛋白表达 .结论 pEF HCVC重组质粒可以在SP2 / 0细胞中长期稳定表达 ,也可以在BALB/c小鼠浆细胞瘤内表达 .
Objective To observe the expression of HCVC gene in SP2 / 0 cells and tumor-bearing mice.Methods Recombinant plasmid pEF HCVC was constructed and transfected into SP2 / 0 cells, and then screened by G418 to establish SP2 / 0 cell line.And the transfected SP2 / 0 cells were subcutaneously inoculated into BALB / c mice to produce plasmacytoma.Results The transfected cells were detected by dot blot hybridization with HCV mRNA.Immunohistochemistry detection of transient transfection and In addition, positive signal was found in the stained transfected SP2 / 0 cells.Immunohistochemical detection of the tumor tissue showed that there was HCVC protein expression.Conclusion pEF HCVC recombinant plasmid can be stably expressed in SP2 / 0 cells for a long time, Can also be expressed in BALB / c mouse plasmacytoma.