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目的：建立测定西替利嗪尿药浓度的反相离子对高效液相色谱法（ＲＰ－ＨＰＬＣ）。方法：以Ｎｏｖａ－ｐａｋＣ１８、４μｍ色谱柱为分离柱，流动相为乙腈∶磷酸盐缓冲液（０．０２ｍｏｌ·Ｌ－１、ＮａＨ２ＰＯ４）∶三乙胺（５０∶５０∶０．１５，Ｖ／Ｖ），ｐＨ值３．０７，内含十二烷基硫酸钠（ＳＤＳ）０．０１０５ｍｏｌ·Ｌ－１，去氯羟嗪为内标，ＵＶ检测波长２２９ｎｍ。结果：方法线性范围为０．２～２０μｇ·ｍｌ－１（ｒ＝０．９９９９），平均回收率为９６．４％±２．３％，日内和日间精密度均小于５％。结论：采用ＲＰ－ＨＰＬＣ法测定盐酸西替利嗪片的正常人尿药浓度，简便，迅速，准确
Objective: To establish a reversed-phase ion-pair high performance liquid chromatography (RP-HPLC) for the determination of urinary drug concentration of cetirizine. Methods: Nova-pakC18 column was used as the separation column. The mobile phase consisted of acetonitrile: phosphate buffer (0.02mol·L-1, NaH2PO4): triethylamine (50:50:0.15, V / V ), PH 3.07, containing sodium dodecyl sulfate (SDS) 0.0105mol·L-1, dechlorination as internal standard, UV detection wavelength of 229nm. Results: The linear range of the method was 0.2 ~ 20μg · ml-1 (r = 0.9999). The average recovery was 96.4% ± 2.3%. The intra- and inter-day precision was less than 5%. Conclusion: The determination of urinary drug concentration of cetirizine hydrochloride tablets by RP-HPLC is simple, rapid and accurate